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Analytical Validation of Quantitative Real-Time PCR Methods for Quantification of Trypanosoma cruzi DNA in Blood Samples from Chagas Disease Patients

Acceso Abierto

ID Minciencias: ART-0000025984-30642

Ranking: ART-ART_A1

Idioma: Inglés

Publicado: 27/08/2015

APC (est): No disponible

Abstract:

An international study was performed by 26 experienced PCR laboratories from 14 countries to assess the performance of duplex quantitative real-time PCR (qPCR) strategies on the basis of TaqMan probes for detection and quantification of parasitic loads in peripheral blood samples from Chagas disease patients. Two methods were studied: Satellite DNA (SatDNA) qPCR and kinetoplastid DNA (kDNA) qPCR. Both methods included an internal amplification control. R

Tópico:

Trypanosoma species research and implications

Citaciones:

175

Citaciones por año:

Altmétricas:

Información de la Fuente:

FuenteJournal of Molecular Diagnostics	Cuartil año de publicaciónNo disponible	Volumen17
Issue5	Páginas605 - 615	pISSNNo disponible
ISSN1943-7811	Perfil OpenAlexhttps://openalex.org/S48180145

Enlaces e Identificadores:

Scienti ID	0000025984-30642	Minciencias ID	ART-0000025984-30642	Pmid URL	https://pubmed.ncbi.nlm.nih.gov/26320872
Scholar citations URL	https://scholar.google.com/scholar?cites=1497764847894880153&as_sdt=2005&sciodt=0,5&hl=en	Oaipmh URL	https://repository.urosario.edu.co/oai/request?verb=GetRecord&metadataPrefix=dim&identifier=oai:repository.urosario.edu.co:10336/26203	Uri URL	https://repository.urosario.edu.co/handle/10336/26203
Open_access URL	https://doi.org/10.1016/j.jmoldx.2015.04.010	Dspace URL	https://repository.urosario.edu.co/handle/10336/26203	Openalex URL	https://openalex.org/W1901605865
Pmcid URL	https://www.ncbi.nlm.nih.gov/pmc/articles/4698797	Pdf URL	https://www.sciencedirect.com/science/article/pii/S1525157815001294	Doi URL	https://doi.org/10.1016/j.jmoldx.2015.04.010
Scholar URL	https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=info%3AmV_XkzEhyRQJ%3Ascholar.google.com&btnG=

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