The passion fruit and other passion flower crops in Colombia regularly are affected by scab, a degenerative disease that attacks different parts of the plant, however the etiology of the disease is not completely known, which led to do this research to establish the etiology of the disease through pathogenicity tests and morphological characteristics of the causal agent, complemented with molecular characterization using the amplified rDNA ITS region and Actin region gen, restriction enzymes and sequencing, concluding that Cladosporium cladosporioides is the causal agent of passion fruit scab in Colombia.