Blood indicators are used as a tool for diagnosing and estimating the severity of metabolic disorders. This work investigates the relationship between blood indicators of lipomobilization and hepatic function in high yielding dairy cows. Two groups of Holsteincows were studied: 27 early-lactating cows (Group 1) and 14 mid- lactating cows (Group 2). The animals originated from four different herds with similar husbandry characteristics. Blood samples were used to determine concentrations of beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), triglycerides (TG), and other serum components as well as the activity of AST and GGT enzymes. The first group had significantly higher BHB and NEFA levels. High lipomobilization and subclinical ketosis were detected in 67% and 41% of early-lactating cows and 14% and 36% of mid-lactating cows, respectively. NEFA tests were not as sensitive as BHB to diagnose subclinical ketosis. Low TG concentrations were observed in all cows suffering from subclinical ketosis and in 61% of those with high lipomobilization. AST values revealed that 30% had hepatic lipidosis in early lactation. Furthermore, lower concentrations of glucose, total protein, and urea suggested that hepatic function was compromised.