Summary A rust disease was observed during routine disease surveys of Eucalypt species in Colombia. The cause of the disease was identified, using morphology and DNA sequence data, as the myrtle rust pathogen, Puccinia psidii . We evaluated the susceptibility of Eucalyptus grandis and the hybrid Eucalyptus urograndis to P. psidii . This is the first report of this pathogen on Corymbia citriodora and Eucalyptus species in Colombia.