The growth plate is a cartilaginous structure located in the metaphysis of long bones, characterized histologically by its stratification and columnar arrangement. It is responsible for assuring longitudinal growth. Evaluation of growth plate histological characteristics has been traditionally performed using qualitative observation; however, some quantitative approaches have been reported using complex techniques. Here, we propose a simple quantitative images based analysis in order to evaluate objectively columnar arrangement within growth plate. For this, we defined six descriptors that were condensated in a geometric tensor. This tensor could be used as a single parameter to evaluate the growth plate organization. Validation of the tensor was performed with growth plate microphotographs of three healthy species (rat, pig and rabbit) and an abnormal one (Csf1 tl /Csf1 tl rat) found in specialized literature. According to our results, the descriptors and the tensor give a complete picture of the organization of the growth plate, reflecting the expected stratification and columnar arrangement of the cells within the tissue. This methodology could be a reliable tool for evaluation of growth plate structure for research and diagnostic purposes, taking into account that it can be easily implemented.