Abstract Currently, plants have gained widespread interest as a source of natural sunscreen. Specifically, plants from high altitude ecosystems are exposed to high UVR levels; therefore, they must produce an adaptive chemical response. The aim of this study was to evaluate the photo‐protection and antioxidant capacity in vitro of nine plants from high altitude ecosystems in Antioquia, Colombia ( Sphagnum meridense , Calamagrostis effusa , Lycopodiella alopecuroides , Morella parvifolia , Baccharis antioquensis , Pentacalia pulchella , Castilleja fissifolia , Hesperomeles ferruginea and Hypericum juniperinum ). B. antioquensis and P. pulchella extracts showed the best results over a broad spectrum UVA ‐ UVB with antioxidant capacity in vitro . However, B. antioquensis extracts presented the highest absorption coefficient in UVB ‐ UVA range among plants under study. Furthermore, the gel formulation containing the crude extract of B. antioquensis showed significant values of UVAPF , UVA / UVB ratio, critical wavelength ( λ c ) and SPF (3, 0.78 380 nm and 4.73 ± 0.26; respectively), indicating interesting photostability and antiradical capacities. All of these properties could be improve in order to satisfy the requirements for broad‐spectrum UVB / UVA protection. Finally, P. pulchella and B. antioquensis extracts could be a potential source of a new natural sunscreen compounds with photostable and antiradical properties.