Objetive : To isolate, to identify and to elucidate the secondary metabolites present in the same fraction extract ethyl acetate of air parts of Aciotis polystachya (Bonplan) Triana (Melastomataceae). Methodology : Techniques using column chromatography, thin layer chromatography and preparative chromatography was isolate, identify and purify secondary metabolites, the analysis of the spectra of 1 H-NMR, 13 C-NMR and two-dimensional NMR techniques ( 1 H- 1 H COSY and HMQC) allowed us to elucidate the chemical structure of these metabolites. Results : The methanol extract was performed Aciotis polystachya solid to liquid extraction with ethyl acetate, fractions 57-64 from the chromatography of the extract usually ethyl acetate, eluted with solvent dichloromethane-ethyl acetate in volumetric proportions 40-60%, 0.03 grams was isolated as a yellow solid after successive chromatographies, similarly fractions 65-73 and 57-64 from the same chromatography was obtained 0.13 grams of total product as yellow crystals. Conclusions : Quercetin and Kaempherol are new secondary metabolites report both gender as this species, and are responsible for many biological and pharmacological activities, which justify some ethnobotanical uses of this plant.