There are normative data of the quality of life (QoL) questionnaire EORTC QLQ-C30 (the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire) for several European countries and Korea, but not for Latin America. The aim of this study was to provide these normative values for the general population in Colombia and to assess differences in terms of age and sex. For that reason, a sample of 1500 representatively selected individuals of the Colombian population completed the EORTC QLQ-C30 form. Results showed that mean scores of the Colombian population are similar to those obtained in European countries; whereas the mean values from a Korean study were lower (worse QoL). Age and sex differences were found in several scales and symptom items. Linear regression analyses were calculated to help quantify the influence of age and gender on QoL. Men reported better functioning and less symptoms than women on all scales, and older individuals reported worse global scores in terms of functioning and lower QoL than younger ones. In sum, the normative values presented can be used to assess QoL scores of Latin American cancer patients and to compare groups of patients with unequal age and sex distributions.