Habitually, control of biological fouling includes application of paints containing toxic substances that end upcontaminating marine ecosystem. Many organisms prevent settlement of other species synthesizing secondarymetabolites that could be used in the elaboration of environmentally friendly anti-fouling paints. This work evaluated the behavior of anti-fouling paints based on extracts from marine invertebrates in the ColombianCaribbean: Agelas tubulata, Myrmekioderma gyroderma, Oceanapia peltata, Aplysina lacunosa, Neopetrosia próxima,and Holothuria glaberrima. The painted panels were submerged in the port of Mar del Plata (Argentina); after 90 days in the sea signi!cant differences were registered in the total coverage between the painted panels andthe controls (p<0.05). The results obtained represent important progress toward using natural compounds incontrolling encrustations.