We document a male juvenile specimen of the vespertillionid bat Eptesicus chiriquinus , recovered on August 15, 2013 at the Autopista del Cafe, El Roble, Quindio, apparently hit by a car. The specimen corresponds to the first record of the Coleccion Mastozoologica de la Universidad del Quindio (CMUQ), identified with catalogue number CMUQ-001. Despite the species has been already reported for the department, the herein documented specimen constitutes the first verified record through a scientific procedure in a peer-reviewed publication. Finally, we discus on the importance of the beginning of an official collection of mammals at the Universidad del Quindio.