This database presents a compilation of previously published and unpublished data on the chemical composition of over 200 Pre-Columbian gold and gold-alloy artefacts recovered in the archaeological region inhabited by the Muisca of Colombia (AD 600-1800), in addition to 35 analyses of geological gold from Colombia. The chemical data have been supplemented with information such as museum reference codes, dimensions, typologies and dates where available, as well as aspects of the artefacts’ manufacture documented by macroscopic observation and metallographic studies. Where possible, the database also includes information relative to the specific location where the artefacts were found and their contextual associations, for example in groups of artefacts recovered as a single offering. The data allow an overview of typological, chemical, and technical aspects of this goldworking tradition but also detailed analyses on a contextual or local basis, as well as comparisons with other regions.