Based on literature and field data, a list of the herpetofaunal in the Departament of Valle del Cauca, Colombia was elaborated. Currently, the known information about amphibian richness is composed of 162 species; most of them frogs and toads (148 anuran species vs 4 salamanders and 10 Caecilians). The reptiles are represented by 130 species, from which 68 are snakes, 53 lizards, 7 turtles and only two are crocodilians. Most of the herpetofaunal of the Valle del Cauca is concentrated on the Pacific region and the Western Andes. The available information indicates that the threatened status of the herpetofauna in the Valle del Cauca is higher for amphibians than reptiles.