Anoplosyllinae possesses palps basally fused, without central surcus, three antennae, and two pairs of tentacular cirri, pharynx straight, short and unarmed. Antennae, tentaculars cirri, and first dorsal cirri smooth, the rest can be smooth or semiarticulate. Eusyllinae possesses palps fused, partially or free, nuchal organs often large, distinct, one or two pairs of tentacular cirri, dorsal cirri variable in length, usually smooth, ventral cirri present, pharynx usually straight, with teeth or unarmed. The objective of this study was to check type and non-type material of the species recorded from the Grand Caribbean. One hundred and eighty six specimens were examined; 135 collected from the Mexican Caribbean, and 51 deposited in museums; in total 9 genera and 17 species of both subfamilies were examined. Type material included Odontosyllis luminosa San Martin, 1990; Opisthodonta luquei (San Martin, 1990); Perkinsyllis spinisetosa (San Martin, 1990); Syllides caribica Licher, 1996; S. gomezi Sail Martin, 1990, and S. papillosa Hartmann-Schroder, 1960. Non-type specimens included Amblyosyllis algefnae Viguier, 1886; A. dorsigera Claparede, 1864; A. madeirensis Langerhans, 1879; Eusyllis kupfferi Langerhans, 1879; Odontosyllis detecta Augener, 1913; O. fulgurans (Audouin and Milne-Edwards, 1883); Sinmerosyllis lamelligera (Saint-Joseph, 1886); Syllides edentulus Claparede, 1868; S. fulvus (Marion y Bobretzky, 1875, and Westheidesyllis gesae (Perkins, 1981).