Dengue is a disease of public health interest for Colombia due to its impact on morbidity and mortality. This disease mantains an endemic behavior in the departments of the country located below 2200 meters above sea level. Between the years 1978 to 2010 it was recorded approximately a number of 1,011,852 cases of dengue in Colombia. Vector populations of Aedes aegypti in the country have been continuously pressed for over five decades with insecticides, being the most widely used tool for interrupting transmission of dengue virus during outbreaks and epidemics in the absence of an available vaccine (Figure 1). The selection pressure excercised with these chemicals has led to the emergence of resistant populations of the vector to organochlorine, organophosphate, pyrethroid and carbamatelike molecules.