We report a new locality for Agalychnis psilopygion (Cannatella 1980) in Biological Research Permanent Plot ( PPIB ), located in the center of the department of Choco; all three individuals were found associated with a permanent water body product human intervention in leaves of bushes , sharing habitat with A. spurrelli , A. aff terranova , Hypsiboas pellucens , H. rubracylus, Craugastor fitzingeri, Dendrosophus phleobodes and Lithobates vaillantis . This record extends the geographic range for the species about 190 km from the type locality. The discovery of this species listed by the Convention on International Trade in Endangered Species of Fauna and Flora in Appendix II also lacks ecological, biological and phylogenetic relationships in the area of influence of the PPIB in the village of Salero is important in terms of conservation of forests for the preservation of amphibians of the Colombian Pacific lowlands.