Objective: to establish the internal consistency and dimensionality of the AUDIT among Colombian native Amerindians dwelling in Bogota, Colombia. Method: a validation study was carried out. A total of 184 Amerindians, 99 men and 85 women. The mean of age was 32.0 years (SD=14.0), and mean of formal scholarship, 6.1 years (SD=3.7). The correlations between items and total score, internal consistency (Cronbach alpha and McDonald omega), and dimensionality (factor structure) were computed. Results: the correlations between items and total score were between 0.368 and 0.788. The AUDIT showed Cronbach alpha of 0.879, McDonald omega of 0.886, and one-dimensional structure that accounted for 50.6% of the total variance. Conclusions: the AUDIT shows excellent internal consistency and one-dimension structure among Amerindians dwelling in Bogota, Colombia.