Resumen Se estudio el efecto de la extrusion asistida y no asistida para la produccion a gran escala sobre el tamano, forma y desempeno bioquimico de capsulas de lacasa-alginato. Las capsulas se formularon usando una concentracion de alginato al 1.0% y 3.0% (m/v) y se prepararon por un metodo de extrusion-goteo. Los resultados mostraron que la tecnica de extrusion afecta el tamano de las capsulas y su distribucion de tamano, pero no afecta su forma. La caracterizacion bioquimica mostro un comportamiento similar entre ambos metodos de extrusion. Sin embargo, las capsulas no asistidas presentaron mayor variabilidad en su actividad enzimatica y menor estabilidad en el tiempo. Los resultados muestran que con el metodo de extrusion, material particulado altamente homogeneo mejora bioprocesos facilitando el control de etapas de temperatura y pH, por lo tanto, un metodo asistido presenta varias ventajas para la produccion de material particulado de lacasa en grandes cantidades. Abstract The effect of assisted and not-assisted extrusion for large scale production on size, shape and biochemical performance of the laccase-alginate beads was studied. The alginate beads were formulated using 1.0% and 3.0% (w/v) alginate concentrations and were prepared by an extrusion-dripping method. Results showed that the extruding technique affects the beads size and size distribution but not the beads shape. The biochemical characterization showed a similar performance for both extruding methods. However, not-assisted beads presented higher enzymatic activity variability and lower stability in time. Results shown that in extrusion method, bioprocesses are improved by highly homogeneous particulate material easing temperature or pH controlled steps, then, an assisted method presents several benefits for producing laccase particulate material at large quantities.