The conditions and characteristics of biobutanol production in ABE fermentation were studied. A cell growth kinetic model structured according to the proposed metabolic pathway Embden-Meyerhof-Parnas (EMP) pathway for the Clostridium Saccharoperbutylacetonicum N1-4 ATCC13564 reaction scheme was used in a continuous fl ow stirred tank. A sensitivity analysis and optimization based on the decision variables butanol productivity, glucose to butanol yield and glucose global conversion for different feed concentrations and dilution rate were performance. Productivity values of 27,46 mM h-1, yield of 0,65 mmol of butanol per mmol of glucose and fi nal conversion of 95,38% at optimal food concentration of 295 mM and optimal fi nal dilution rate of 0,15 h-1 were obtained.