Electrochemical methods were used to promote and evaluate the oriented adsorption of albumin and collagen on polymer surfaces of polylactic acid by applying an anodic overpotential in the range of 0 V to 0.45 V. The adsorption was studied using simultaneous measurements of electrochemical impedance spectroscopy (EIS) and quartz crystal microbalance (QCM) in a temperature range of 25°C to 45°C, and protein variations between 5 and 20 mg/ml phosphate buffer solution of pH 7,4. Increasing protein concentration in the electrolyte reduces resistance to charge transfer associated with the interaction between protein and electrode, thus showing a greater adsorption on the surface, confirmed by the increased mass measured by QCM. With the electrochemical polarization, the hydrophilicity of PLA coating was improved, showing a higher affinity and adsorption capacity with collagen-rich solutions.