Standardising a simple protocol for extracting yeast from genomic DNA Resumen: Se estandarizo un protocolo rapido, sencillo y de bajo costo para la extraccion de ADN genomico de levaduras a partir de lisis de la pared celular mediante tratamiento enzimatico y precipitacion por alcoholes. El empleo de la enzima Beta-glucoronidasa en reemplazo de la enzima Zimolasa, permitio obtener ADN en alta concentracion (124,9±30,2 ng/I¼l) y de buena calidad (A260/A280 nm =1,86±0,1), ideal para su uso en estudios de biologia molecular. Ademas, se adiciono un paso de incubacion del ADN obtenido a 100° C para inactivar ADNasas. La calidad del ADN obtenido fue evaluada por medio de la amplificacion de la region ITS1-5.8S-ITS2, presentando bandas definidas y cuantificables (entre 380 y 880 pb) ideales para estudios de identificacion molecular y filogenia. Palabras clave: levadura; extraccion de ADN; Beta-Glucoronidasa. Abstract: A quick, simple and low-cost protocol for extracting genomic DNA from yeast by cell wall lysis involving enzymatic treatment and alcoholic precipitation was standardised. Higher DNA yields (124.9±30.2 ng/I¼l) were obtained by using beta-glucuronidase instead of zymolyase; these had very high quality (A260/A280 nm = 1.86±0.1) and would be suitable for use in molecular biology assays. Moreover, a DNAse inactivation step was also introduced by incubation at 100 °C to further ensure DNA stability. DNA quality was assayed by PCR amplification of the ITS1-5.8S-ITS2 region, revealing defined, quantifiable 380 to 880 bp bands. These results show that the protocol is ideal for molecular identification and phylogenetic studies. Key words: Yeast; DNA extraction; beta-glucoronidase.