The study of culicine mosquitoes (Diptera: Culicidae) in Colombia, has been limited even though this subfamily is important in public health. This study aimed to determine the species of Culicinae breeding in stands of Guadua angustifolia in areas with coffee plantations. Three breeding sites were distinguished: guadua stumps, perforated internodes and containers. Nine mosquito species were identified which two are new records for Colombia (Orthopodomyia albicosta, Wyeomyia oblita), five new records for the departments (Culex secundus, Cx. antunesi, Limatus durhami, Trichoprosopon digitatum and Sabethes undosus). Two others are under current study (Trichoprosopon sp. part of the Pallidiventer complex, and Toxorhynchites sp.). There was a relationship between the species found and the water volume and height above ground, measured for each of the breeding sites. Two of the species, Tr. digitatum and Li. durhami, are reported in literature as potential vectors of arboviral agents; Toxorhynchites is grouped with species whose larvae are predators. The results support an important role for guadua as breeding sites for various mosquitoes, including some species of public health importance.