The ventilatory threshold (VT) is the point at which ventilation intensifies disproportionately concerning to the oxygen uptake, this parameter is essential within the training methodology to optimize performance; the purpose of this work is to measure the degree of agreement between the determination of the ventilatory threshold determined by for oxygen uptake and four methods by Heart Rate Variability (HRV).Methodology: Twelve professional female cyclists between 18 and 28 years of age were evaluated through a maximal incremental test on a cycle ergometer; gas exchange was measure with a portable telemetric device (Cosmed K4b2 ® ) and simultaneously the HRV was registered by R-R recording trough a Polar RS800CX ® (Polar, Finland) heart rate monitor.Statistics: The degree of precision and accuracy established between the variables mentioned and normality test Shapiro-Wilk.Results: rho = 1.00 with pvalue < .05,and Lin correlation and concordance coefficients of .99 with r = 0.98 for ventilatory threshold 2 (VT2), constituting a level of precision and accuracy almost perfect; by contrast, ventilatory threshold 1 (VT1) did not show significant precision and accuracy.This study suggests that VT2 can be identified from The time series.RR using the analysis of frequency and temporal domains likewise allows.Us to have a reference measure for future research in other sports.