Objective. This study aimed to characterize the symptoms of musculoskeletal disorders (MSDs) and their related factors in a group of orthodontists in the city of Medellín (Colombia). Methods. A cross-sectional study was conducted in a sample of 100 orthodontists. A survey provided sociodemographic and work-related information. MSDs were recorded through the Nordic questionnaire. Descriptive and bivariate analyses were carried out by determining the prevalence of MSD symptoms by anatomical zone and selected variables. Results. Prevalence of MSDs was 81% for males and 88% for females. Anatomical regions were the neck (males 44%, females 59%), shoulders (males 44%, females 48%), lower back-lumbar zone (males 63%, females 51%), elbow-forearm (males 15%, females 15%) and wrist-hand (males 32%, females 51%). The frequency of symptoms was higher in males >41 years old, with more than a work contract or females who worked >40 h per week, and in orthodontists (males-females) who reported higher work-related dissatisfaction levels. Conclusions. A high prevalence of MSDs was found among orthodontists, with differences in the frequency of symptoms related to sociodemographic and work-related factors. Preventive strategies related to health and work safety would be advisable considering the particular needs of this dentist group.