It has been established that organic acid secretion by rhizosphere microorganisms is one of the mechanisms to solubilize the phosphorus (P) attached to insoluble mineral compounds in soil. This action is an important biotechnological alternative, especially in those soils where high fixation of this nutrient occurs, a very common situation in the tropics. This research evaluated the ability performed by five bacterial and five fungal isolates from Typic Melanudands soil to produce organic acids and generate available phosphorus from insoluble P sources. Given these concerns, the selected microorganisms were replicated for 7 days in liquid medium Pikovskaya (PVK) modified sources tricalcium phosphate (P-Ca), aluminum phosphate (P-Al) and iron phosphate (P-Fe). The results indicated that phosphorus availability in the media, correlates positively with the organic acids production in each of the sources used (P-Ca (0.63), P-Al (0.67) and P-Fe (0.63). In turn, the chemical processes linked to the phosphates solubilization (e.g., Ca availability) affected the development of the microorganisms tested. Both, fungi and bacteria varied in their ability production and type of metabolized organic acids, the most frequent were as follows: citric and gluconic acid.