This paper analyzes the link between residential segregation and human capital composition in neighborhoods of Cali (Colombia).The empiric strategy starts with the calculation of local and global indexes using census data for neighborhoods by 1993 and 2005.The evidence suggest the existence of spatial clusters: highly skilled persons locate in longitudinal central zone, while the black community with low educational level locates in the suburbs of the city.The paper shows a number of interesting results: identify hot and cold spots or very homogeneous areas of the city, describes the changes in residential segregation in Cali over time and, finally, the paper contrasts the hypothesis of persistence in the segregation through spatial econometrics models.Results show that there is a great deal of spatial persistence in segregation.