The present study is an updated revision of the richness, composition and distribution of amphibians and reptiles in Casanare. A total of 1,012 specimens from the main Colombian biological collections were examined and 658 specimens were collected in four Casanare municipalities. As a result, we reported 46 species of amphibians and 65 species of reptiles of which six are new records and two extend their known distribution in Colombia. Additionally, we identified Sácama, Támara and Recetor as the municipalities with the poorest knowledge of their amphibians and reptiles.