Guava roots are parasitized by the root knot nematode (Meloidogyne spp.), which causes production losses between 30 and 60%. Due to this, the main goal of this research was to evaluate the reaction of four varieties of guava under nursery conditions to the attack of Meloidogyne incognita and M. javanica complex. In the Jaibana Frutales nursery, in the municipality of Pereira (Risaralda), seedlings of four varieties of guava: Guava pear and Common guava (Psidiumguajava), Cas guava (Psidiumfriedrichsthalianum), and Peruvian guava (Psidiumcattleianum), were planted in plastic bags containing soil and rice hull in a 3:1 proportion. Twenty days after planted, 15 seedlings of each variety were inoculated with a mixture of 1,000, 5,000 and 10,000 eggs and Meloidogyne spp juveniles, and their respective controls without inoculation. Seedlings were assigned under a completely randomized design. The Peruvian guava variety with 5.75g and 8.03g roots and aerial parts dry weight respectively, and Cas guava variety with 10.27g and 9.23g roots and aerial parts dry weight, respectively, had the lowest root gall index with 0.1 and 1.1, and Meloidogyne spp. reproduction with values between 75 and 4.575 eggs and juveniles/100g of roots. These varieties were statistically different from the guava Pear variety with 13,96g y 11,33g roots and aerial parts dry weight respectively, and Common guava with 11,35g y 13,9g roots and aerial parts dry weight respectively, which had the highest root gall index with 5 and reproduction with 149.775 eggs and 72.950 juveniles/100g of roots. In conclusion, the Cas and Peruvian varieties were resistant and immune respectively, to Meloidogyne incognita and M. javanica, allowing them to be used as rootstocks or patronages, while guava Pear and Common guava were highly susceptible to both species.